科研动态

为实现袋鼠临床样本中疱疹病毒1型（Macropodid herpesvirus 1，MaHV-1）的出入境快速检测，基于MaHV-1的gB基因序列设计特异引物和TaqMan探针，建立了一种MaHV-1荧光PCR检测方法。试验结果显示，该方法只对MaHV-1 gB基因呈现特异性扩增，与禽传染性喉气管炎病毒、伪狂犬病毒和牛传染性鼻气管炎病毒不发生交叉反应，对阳性标准质粒对照（pCR-MaHV-1-gB）的最低检测限为8个拷贝数/反应。该方法的组内和组间试验的Ct值变异系数介于0.17%~0.96%之间，具有良好的重现性。试验结果表明，本研究建立的实时荧光PCR方法可用于袋鼠MaHV-1的病原学检测。

Development of a Real-time PCR for Detection ofMacropodid Herpesvirus 1

For rapidly detecting macropodid herpesvirus 1（MaHV-1）in clinic samples from kangaroos at entry-exit ports，a real-time PCR was developed by designingprimers and probes based on the sequence of gB gene. The results showed themethod could only amplify the MaHV-1 gB gene and no cross-reaction with otherkinds of virus was observed，including infectious bovine bronchitis virus，avian laryngotracheitis virus andpseudorabies virus. The detection limit was 8 copies/reaction for the positivestandard plasmid control（pCR-MaHV-1-gB）. The coefficients of variation（CV）of intra-assay and inter-assay were between 0.17 % to 0.96 %，showing good repeatability. In conclusion，the established method was applicable forpathogenic detection of MaHV-1 from the kangaroos.

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