科研动态

为了分析弓形虫GT1虫株GRA15（GRA15GT1）蛋白的反应原性，通过PCR扩增编码GRA15GT1 52~635氨基酸肽段的基因片段，构建pGEX-6P-1-GRA15GT1载体，转化BL21菌诱导表达；通过SDS-PAGE和Western blot方法进行表达验证及反应原性分析。结果显示：SDS-PAGE及以GST标签抗体为一抗进行Western blot，均有目的条带，比理论值稍大；以猪弓形虫阳性血清为一抗的Western blot条带与GST抗体孵育后的条带大小一致。上述结果表明，GRA15GT1蛋白具有较好的反应原性，这为下一步分段表达GRA15GT1蛋白，研究其在弓形虫血清学分型中的应用奠定了基础。

Expression and Immunoreactivity Exploration of GRA15Protein

of Toxoplasma gondii GT1 Strain

In order to analyze the immunoreactivity of GRA15protein of Toxoplasma gondii GT1 strain（GRA15GT1），the gene encoding a 584-residue peptide（from aa52 to aa635）was amplified by PCR，then the pGEX-6P-1-GRA15GT1 vector wasconstructed and transformed into BL21 strain of E. coli. After inducibleexpression，the purified GRA15 protein were obtainedand verified by SDS-PAGE and Western blot. Results showed that，the target strip was slightly larger thanits estimated size when the GST-tag mouse monoclonal antibody was used asprimary antibodies，as well as the swine anti-T. gondii positive serum. The resultsindicated GRA15GT1 protein had good immunoreactivity，which would lay a foundation for the next stepof GRA15GT1 protein expression and its application in serological typing ofToxoplasma gondii.

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