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为了解鸡群中I群禽腺病毒(FAdV-I)的流行现状,从山东、黑龙江、吉林等地部分大规模养鸡场采集病料,进行病毒分离、PCR鉴定及同源性分析。结果显示:从76份病料中分离到10株FAdV-I,分离率为13%;10株FAdV-I中,1株为血清4型,6株为血清8b型,1株为血清9型,2株为血清11型。结果表明,山东、黑龙江、吉林等地均存在一定的FAdV-I流行,主要流行血清型已由4型转变为8b型,因此需要评估当前的4型灭活苗能否有效防控这些地区的FAdV-I流行。
Isolation and Identificationand Homology Analysis on Group I Fowl Adenovirus
In order to recognize the prevalence ofgroup I fowl adenovirus(FAdV-I)in poultry,samples were collected from several large-scalechicken farms in Shandong,Heilongjiang and Jilin provinces for FAdV isolation,PCR assay and homology analysis. Resultsshowed that 10 FAdV-I strains were isolated from 76 samples with the positiverate reaching 13%. Among the 10 FAdV-I strains,1 of them belonged to serotype 4,6 of them belonged to serotype 8b,1 of them belonged to serotype 9,and 2 of them belonged to serotype 11. Theresults indicated that certain FAdV-I infection existed in Shandong,Heilongjiang and Jilin provinces,and the main serotypes had been transferredfrom serotype 4 to serotype 8b,thus the current serotype 4 inactivated vaccines need to be evaluatedto determine if they could control the spread of FAdV-I effectively.
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