科研动态

为了解我国猪肺炎支原体种群结构和进化关系，2018—2019年采集山东、广西、河南、江西、云南、天津、湖南等地猪组织样品1 242份，进行猪肺炎支原体荧光定量PCR和巢式PCR检测，并对测序确认的87份阳性样品，采用adk、rpoB、tpiA 3个管家基因作为分型参考进行多位点序列分型（MLST），并对3个管家基因均扩增成功的阳性样品进行p146基因分型，分析我国猪肺炎支原体种群结构，并结合pubmlst数据库公布的151个序列类型（ST）进行eBURST分析。结果显示：有24份阳性样品的3个管家基因全部扩增且测序成功，共分为10个ST，均为国内外首次报道；10个ST分为3个克隆复合体（CC）和4个独特型，其中ST128占41.7%（10/24），样品来自江苏、河南和广西，为本次检测的主要流行型。结果表明，我国猪肺炎支原体分布较广，具有一定的基因型独特性。本研究为我国猪肺炎支原体的流行病学数据库研究提供了新资料，有助于掌握国内猪肺炎支原体基因型分布情况，进一步完善了猪肺炎支原体流行病学数据库。

Multi-locus Sequence Typing of Mycoplasma hyopneumoniae in Some Regions of China during 2018 to 2019

In order to investigate the genetic characteristics and evolutionary relationship of Mycoplasma hyopnumoniae（M. hyopnumoniae）in China，1 242 swine tissues were collected during 2018 to 2019 from Shandong，Guangxi，Henan，Jiangxi，Yunnan，Tianjin and Hunan for detection of M. hyopnumoniae by real-time PCR and nested PCR，and 87 positive samples were confirmed. DNA was extracted from positive samples and classified according to p146 gene and multi-locus sequence typing（MLST）with adoption of 3 housekeeping genes including adk，rpoB and tpiA，then the population structure of M. hyopnumoniae in China was analyzed，and eBURST analysis was carried out in combination of 151 STs published in pubmlst database. The results showed that all the three housekeeping genes of 24 positive samples were successfully amplified and sequenced，which were divided into 10 STs and firstly reported in the world，involving three clone complexes（CCs）and four singletons，in which，ST128 was prevalent and accounted for 41.7%（10/24）and the samples were collected from Jiangsu，Henan and Guangxi. It was concluded that M. hyopnumoniae spread widely with unique genotypes to some extent. Therefore，some new data was provided for the study on epidemiological database of M. hyopnumoniae，which contributed to the identification of its distribution and further enriched relevant database.

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