科研动态

牛结节性皮肤病（lumpy skin disease，LSD）是由牛结节性皮肤病病毒（lumpy skin disease virus，LSDV）引起的急性、亚急性传染病。我国于2019 年8 月在新疆伊犁地区首次确诊该病。快速准确检测是防控该病的关键。目前，应用常规PCR、实时荧光定量PCR、高分辨率熔解曲线分析、环介导等温扩增等分子检测方法，已实现了LSDV与其同属不同种病毒的通用检测和鉴别检测以及LSDV野毒株与疫苗株的区分；应用病毒中和试验、酶联免疫吸附（ELISA）和蛋白印迹试验（WB）等免疫学方法，可进行羊痘病毒属的特异性抗原与抗体检测，而对于LSDV特异性抗体检测方法尚有待研究。LSD作为一种新传入我国的外来动物疫病，有必要运用快速准确的分子或免疫学检测方法加强监测。本文概述了LSD诊断方法研究进展，为我国LSD诊断技术研发提供参考。

Research Progress on Diagnostic Methods of Lumpy Skin Disease

Lumpy skin disease（LSD），an acute and subacute infectious disease，is caused by lumpy skin disease virus（LSDV）. In August 2019，Chinese first case was confirmed in Yili Prefecture，Xinjiang. To prevent and control the disease is subject to rapid and accurate detection. Presently，the goal of generic detection of LSDV and its congeneric virus of different species as well as differential diagnosis of wild strains and vaccine strains have been achieved by a series of molecular detection methods，such as conventional PCR，real-time fluorescent quantitative PCR，high-resolution melting（HRM）curve analysis，loop mediated isothermal amplification（LAMP）；the specific antigen and antibody of capripoxvirus could be detected by the immunological methods，such as virus neutralization test（VNT），enzyme-linked immunosorbent assay（ELISA），Western Blot（WB），etc. However，the detection methods for specific antibodies against LSDV still need further studies. As a new exotic animal disease，LSD should be further monitored by rapid and accurate molecular or immunological methods. In conclusion，relevant research progress was summarized for the purpose of providing some references for the development of LSD diagnosis technology in China.

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