科研动态

为探索一次性检测猪肉中多种磺胺类药物残留的检测方法，建立了同时测定猪肉中22种磺胺类药物残留的固相萃取-超高效液相色谱方法。样品用3%乙酸乙腈提取，MCX固相萃取柱净化，水和甲醇淋洗，5%氨化甲醇洗脱，氮气吹至近干，0.1%甲酸-乙腈（9:1，V/V）溶液复溶，二极管阵列检测器270 nm检测。结果显示：该方法对22种磺胺类药物检测的线性关系良好（R2＞0.999），定量限为2.5~10.0 μg/kg，检测限为1.0~4.0 μg/kg；各药物平均回收率为83.2%~96.6%，批内和批间变异系数分别为1.1%~3.5%和3.6%~13.4%。结果表明，该方法灵敏度高、重复性好、成本低廉，且操作简单、快速，可用于猪肉样品中多种磺胺类药物残留的同时检测。

Simultaneous Determination of 22 Kinds of Sulfonamide Residues in Pork by SPE-UPLC

In order to develop a method for simultaneously detecting various sulfonamide residues in pork，a solid-phase extraction-ultra-high performance liquid chromatography（SPE-UPLC）method for detecting 22 kinds of sulfonamide residues in pork was established. Samples were extracted by 3% acetic acid acetonitrile，purified by MCX solid phase extraction column，rinsed by water and methanol，eluted by 5% ammoniated methanol，dried by blowing with nitrogen，and redissolved by 0.1% formic acid-acetonitrile（9:1，V/V）solution，and then detected by a diode array detector at 270 nm. The results showed that the linear relationship was good（R2>0.999）for detection of the 22 kinds of sulfonamides by the method，with the limit of quantification of 2.5 to 10.0 μg/kg，the detection limit of 1.0 to 4.0 μg/kg；the average recovery rate of each drug ranged 83.2% to 96.6%，and the variable coefficients within and between batches were 1.1% to 3.5% and 3.6% to 13.4%，respectively. It was concluded that various sulfonamide residues in pork could be simultaneously detected by the established method due to its advantages of high sensitivity，good repeatability，low cost，simple and rapid operation.

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