科研动态

为了解湖南猪群猪圆环病毒1型（PCV1）的遗传变异情况，以PCV1阳性DNA为模板，使用PCR方法分两段扩增PCV1全基因序列并进行拼接。结果共获得5个PCV1全基因组，其中3株全基因大小为1759 bp，另外2株分别为1758 bp和1760 bp。5株全基因组同源性为98.4%~99.7%，与国内外的PCV1全基因组同源性为98.1%~99.7%；ORF2序列同源性为97.6%~99.6%，与国内外的PCV1 ORF2同源性为96.1%~99.9%。系统发育树显示，5株PCV1均属于同一分支，并显示出一定的地理差异，但差异较小。结果表明，湖南省流行的PCV1毒株基因较为稳定，分离株间差异较小。本研究为湖南省猪圆环病毒病防控及相关研究奠定了基础。

Whole Genome Sequencing of Five Strains of PCV1 in Hunan Province and Analysis on Their Genetic Evolution

In order to identify the genetic variation of porcine circovirus type 1（PCV1）in pigs in Hunan Province，taking PCV1 positive DNA as a template，two sections of PCV1 gene sequence were amplified by PCR and then spliced together to form the whole genome. Five strains of whole genomes were obtained，in which，three were 1759 bp，and two were 1758 bp and 1760 bp，respectively. The homology of the whole genome was 98.4% to 99.7%，which shared the homology of 98.1% to 99.7% with other whole genomes of PCV1；the homology of ORF2 sequence was 97.6% to 99.6%，which shared the homology of 96.1% to 99.9% with other PCV1 ORF2. As indicated by the phylogenetic tree，all the five strains belonged to the same branch with slightly geographical difference. In conclusion，the strains were steadily prevalent in Hunan Province with little difference. A foundation was thereby provided for future prevention and control of PCV in Hunan and for relevant studies.

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