科研动态

为了解福建省鸡场圆圈病毒3型（GyV3）的流行及其VP2基因变异情况，2021年在南平市、龙岩市和福州市的36个鸡场，采集365份疑似GyV3感染病鸡的腺胃样品，通过PCR进行GyV3核酸检测。结果显示：共检出GyV3核酸阳性55份，阳性检出率为15.06%。对5份GyV3阳性样品进行VP2基因序列分析发现，5份样品的VP2基因核苷酸和推导氨基酸序列相似性分别为98.6%~99.9%和98.3%~100%，与参考株山东肉鸡分离株SDAU-1和巴西家鸡分离株核苷酸和推导氨基酸序列的相似性分别为98.3%~99.2%和97.5%~99.2%，均属于Group A分支中的GyV3小分支。结果表明：福建省鸡场存在GyV3流行；其VP2基因遗传序列稳定，可作为GyV3感染流行病学监测的首选基因。本研究为GyV3的后续研究提供了理论依据。

Etiological Detection of GyV3 in Some Farms of Fujian Province and Analysis on Its VP2 Gene

In order to investigate the prevalence and VP2 gene variation of gyrovirus 3（GyV3）in chicken farms in Fujian Province in 2021，365 suspected samples of glandular stomach infected with GyV3 were collected from 36 farms in Nanping，Longyan and Fuzhou cities to detect GyV3 nucleic acid by PCR. The results showed that 55 positive samples were detected，with a positive rate of 15.06%. It was found that，by sequence analysis of VP2 gene in 5 GyV3 positive samples，their homology of nucleotide and deduced amino acid sequence of VP2 gene were from 98.6% to 99.9% and 98.3% to 100%，respectively，from 98.3% to 99.2% and 97.5% to 99.2% with those of the reference Shandong broiler strain SDAU-1 and Brazil domestic chicken strain，respectively，all of which belonged to the small branch of GyV3 in group A. In conclusion，GyV3 was prevalent in the farms across the province，and stable genetic sequence was observed in its VP2 gene，which could be used as the first choice for epidemiological surveillance over GyV3 infection. A theoretical basis was thereby provided for future researches on GyV3.

信息来源：https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDAUTODAY&filename=ZGDW202208003&uniplatform=NZKPT&v=vPzqR5W2eDACMu4nN2FrbQUNCucSQCAXG47vSQCRm6GDYHaWc6NGTJAAmXG6Wq6u