科研动态

B2L蛋白是羊口疮病毒（ORFV）的主要囊膜蛋白，为重要保护性抗原之一，可刺激机体产生强烈的抗体反应。本研究对B2L基因运用Visual Gene Developer生物信息学软件，分析其在大肠杆菌、毕赤酵母、昆虫杆状病毒表达系统中的密码子使用偏好性，从而选择一个最适表达系统，以增加B2L蛋白的表达量。分析表明，B2L基因在昆虫杆状病毒表达系统中会得到更好的表达，这为B2L基因表达系统的选择提供了参考。

Analysis on the Codon Preference of B2L Gene of Orf Virus

As the main envelope protein of orf virus（ORFV），B2L protein isone of the important protective antigens，which couldstimulate the organism to produce specific antibodies. In the study，by use of bioinformatics software of Visual Gene Developer，the codon preference of B2L gene in the expression systems of E.coli，Pichia pastoris and insect baculovirus wereanalyzed，and an optimal expression system accordinglywas selected to increase the expression of B2L protein. According to theanalysis，B2L gene could be expressed better in the expression system of insectbaculovirus，which would provide some references forselecting relevant expression system.

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