科研动态

为准确检测猪圆环病毒2型（PCV2）并分析其基因序列情况，在序列比对分析的基础上，设计一对简并引物和一条探针，经体系优化建立了PCV2的荧光PCR方法。该方法可检出10拷贝的PCV2质粒DNA，不与猪瘟病毒等多种病毒发生交叉反应。对送检的2批次45份病死猪脏器进行检测，检出25份阳性样品，与套式PCR方法的复合率为95.6%；对3份强阳性样品使用一对全基因组扩增引物，扩增了PCV2型全基因片段；经克隆测序后分析，发现3个病毒基因分别属于2种基因亚型（PCV2b和PCV2d）。本研究对于PCV2的准确检测及其变异特征的了解具有参考意义。

Detection ofPorcine Circovirus Type 2 in Dead Pigs and Analysis on ItsComplete Genome Sequence

In order toaccurately detect porcine circovirus type 2（PCV2）and to analyze its genome sequence，a pair of degenerate primers and aprobe were designed based on sequence alignment analysis，after systematic optimization，a real-time PCR method was established，which could detect 10 copies ofplasmid DNA of PCV2，with no anycross reaction with classical swine fever virus and other viruses. Then twobatches of 45 organ samples collected from dead pigs were tested by establishedmethod，and 25 samples were detected to bepositive，the coincidence rate with nested-PCRwas 95.6%；for the three strong-positive samples，their complete gene fragments of PCV2were amplified by a pair of primers，then weresequenced and analyzed，and it wasfound that the genes of the three viruses were classified into two differentsubtypes（PCV2b and PCV2d）. Therefore，it was of certain significance toconfirm relevant test results and recognize any variation of PCV2 by the abovemethod.

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