科研动态

为建立一种快速准确检测H9亚型禽流感病毒基因的检测方法，根据GenBank中H9亚型禽流感病毒血凝素编码基因进行荧光检测引物和探针设计，建立了一种针对H9亚型禽流感病毒的荧光RT-PCR检测方法。利用该方法进行灵敏度和特异性检测，并用本方法和国家标准中的荧光RT-PCR检测方法，同时对临床样本进行检测。结果显示，仅H9亚型禽流感病毒出现了正常荧光检测曲线，而其他病毒及阴性对照均未出现；检测灵敏度为RNA终浓度10-4 ng/μL（1.30×104 copies/μL）；临床样本检测结果与国标方法一致，符合率为100%。结果表明，该方法特异性强、灵敏度高，可用于H9亚型禽流感病毒检测。

Establishment and Application of a Real-time RT-PCR Assay for Detection

of H9 Subtype Avian Influenza Virus

In order to establish a method for detecting H9 subtype avian influenza virus（AIV-H9）rapidly and accurately，the fluorescence detection primers and probes were designed and synthesized according to hemagglutinin encoding gene of AIV-H9 registered in GenBank，and a fluorescence PCR assay was thereby established，and its specificity and sensitivity were tested. Then by the established assay and the RT-PCR stipulated in national standard，some clinical samples were simultaneously tested. The results showed that the normal fluorescence detection curve only appeared in AIV-H9 rather than other non-specific viruses and negative sample；its detection limit was 10-4 ng/μL（1.30×104 copies/μL）of RNA；the detection result of clinical samples by established assay was consistent with the national standard，and the coincidence rate was 100%. In conclusion，the assay could be used to detect AIV-H9 due to its strong specificity and high sensitivity.

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